ICSI is an acronym for intracytoplasmic sperm injection which is one of the most successful forms of treatment for men who are infertile and is used in nearly half of all IVF treatments. ICSI only needs one sperm, which is injected directly into the egg. Furthermore, the fertilized egg is then transferred to your uterus to cause a pregnancy. ICSI is a very effective method to fertilize eggs in the IVF lab after they have been aspirated from the female. IVF with ICSI involves the use of specialized micromanipulation tools and equipment and inverted microscopes that enable embryologists to select and pick up individual sperm in a specially designed ICSI needle.The needle is carefully advanced through the outer shell of the egg and the egg membrane - and the sperm is injected into the inner part (cytoplasm) of the egg.